Culture-independent methods for qualitative and quantitative microbiological detection and identification

Lecture for 2nd year undergraduate students who are new to the study of microbiology on «Culture-independent methods for qualitative and quantitative microbiological detection and identification».

Covered methods such as:

1. Polymerase Chain Reaction
   
2. DNA/RNA Hybridization
   
3. Fluoresence in situ hybridization
   
4. Mass spectrometry
   
5. Enzyme-linked immunosorbent assay
   
6. Next Generation Sequencing
   

As well as their advantages and disadvantages.
Each of the above methods was dealt with from a fundamental point of view only, without a detailed discussion of the various modifications of each, due to the introductory and expository nature of the lecture.

Download materials of the lecture