Lecture: Culture-independent methods for qualitative and quantitative microbiological detection and identification

Guest lecture, BINUS University, Food Technology Department, 2023

Lecture for 2nd year undergraduate students who are new to the study of microbiology on «Culture-independent methods for qualitative and quantitative microbiological detection and identification».

Covered methods such as:

  1. Polymerase Chain Reaction
  2. DNA/RNA Hybridization
  3. Fluoresence in situ hybridization
  4. Mass spectrometry
  5. Enzyme-linked immunosorbent assay
  6. Next Generation Sequencing

As well as their advantages and disadvantages.
Each of the above methods was dealt with from a fundamental point of view only, without a detailed discussion of the various modifications of each, due to the introductory and expository nature of the lecture.

Download materials of the lecture